Recombinant Human Osteoglycin Protein, HEK293 Lysate

Proteins|Recombinant Proteins|Human Proteins

Ambient temperature

Recombinant protein has a calculated molecular mass of 32. The actual molecular weight may increase differently due to the potential post-modifications (PTMs).

OGN

Lyophilized from a 0.2 µm filtered solution in PBS containing mammalian cell protease inhibitor cocktail

Osteoglycin, transfected HEK293 cell lysate, Osteoglycin, Osteoinductive Factor, Mimecan Proteoglycan, SLRR3A, OIF, Corneal Keratan Sulfate Proteoglycan, Osteoglycin (Osteoinductive Factor), OG

Unpurified cell lysate. HEK293 cells transfected with expression vectors harboring target gene were harvested and washed with PBS twice. The cell pastes were re-suspended with ice-cold PBS containing mammalian cell protease inhibitor cocktail and further lysed with freeze-thaw cycles. After clarifying with 20, 000 g centrifugation at 4°C for 30 min, the lysate was aliquoted, lyophilized, and stored at -80°C immediately. Protein concentration was determined by BCA kit (Thermo Scientific, Inc.) using BSA as protein standard. The gene overexpression in lysate was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the lysate derived from HEK293 cells transfected with the empty expression vector was used as a negative control.

Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water.