AbFluor™ 555-Phalloidin

Organelle extraction and staining

Cell analysis

Gel pack with blue ice.

Phalloidin belongs to a class of toxins called phallotoxins, which are isolated from the death cap mushroom (Amanita phalloides). It is a bicyclic peptide that binds to F-actin specifically. Therefore, the distribution of F-actin can be very conveniently studied by using a fluorescent dye-labeled phalloidin. Inside the phalloidin, there is an unusual thioether bridge between cysteine and tryptophan, which can form an inner ring structure. When the pH is raised, the thioether is cleaved and the phalloidin loses its affinity for actin.

Lyophilized powder

• Optimized staining protocol for labeling, identifying and quantitating F-actin in formaldehyde-fixed and permeabilized tissue sections, cell cultures or cell-free experiments.
• Proprietary AbFluor™ 555-Phalloidin (Orange Fluorescence) (Ex/Em = 555/565 nm)-high-affinity probe for F-actins and much higher photostability than the other fluorescein-phalloidin conjugates.

AbFluor™ 555-Phalloidin, BMD00083