Anti-Fade Mounting Medium (With DAPI)

Anti-Fade Mounting Medium (With DAPI)

Anti-Fade Mounting Medium (With DAPI) is a glycerol-based mounting medium containing an anti-fluorescence quenching reagent. It can be used to seal tissue or cell slides for fluorescence-related applications. After mounting, slides can be observed for several weeks without drying out or requiring sealing. This medium can help prolong fluorescence and maximise fluoresence intensity, and assist with antigen-antibody binding.

This medium is compatible with a wide range of fluorophores and fluorescent proteins, such as blue fluorescent protein, FITC, Cy2, Cy3, Cy5, Cy7, rhodamine, Texas Red, AMCA, R6G, Hoechst 33258, AF, and more.

This medium contains DAPI, a blue fluorescent DNA stain which binds strongly to ""A-T""-rich regions in DNA, exhibiting up to a 20-fold increase in fluorescence. It can pass through intact cell membranes, and therefore can be used to stain both live and fixed cells for fluorescence microscopy and flow cytometry. It can also be used for counting cells, measuring apoptosis, sorting cells based on DNA content, and as a nuclear segmentation tool in imaging analysis. DAPI has an absorption maxima at 340 nm and an emission maxima at 488 nm. When bound to double-stranded DNA, the absorption maxima changes to 358 nm (ultraviolet) and the emission maxima to 461 nm (blue).

Contains glycerol.