Protein G (Gold, 40nm)

Blue Ice

Affinity Purified

38220090

Conjugation: 40nm colloidal gold particle at 20ug/ml.

Protein G lacking the albumin binding region (avoids undesirable reactions with albumin); Fc binding domain is still present. Each Protein G molecule can bind 2 molecules of IgG, allowing the formation of a precipitate.

Protein G is a small globular cell surface protein produced by Streptococcus sp. It is composed of two or three nearly identical domains of 55 amino acids each. It binds to Fc fragment of IgG with high affinity. Protein G will bind IgGs from many species including all human and mouse subclasses. It does not recognize IgM, IgA, IgE or serum albumin. Protein G binds IgG from rat, , but substantially more weakly than IgG from other species.

Recombinant Protein G is a highly stable surface receptor from Streptococcus sp. Lancefield Group G, produced in Escherichia coli, which is capable of binding the Fc portion of immunoglobulins, especially IgGs, from a large number of species (Boyle and Reis, 1987).

Applications:
Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C. For long-term storage, aliquot and store at 4°C. Do not freeze. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. Gold conjugates are sensitive to light.