SureLight™ B-Phycoerythrin ( B-PE)

Because of its high quantum yield, B-PE is considered the world’s brightest fluorophore.

It is compatible with commonly available lasers and gives exceptional results in flow cytometry, Luminex® and immunofluorescent staining. B-PE is also less “sticky” than common synthetic fluorophores and therefore gives less background interference.

 

 

545nm

Supplied as a functionally water soluble ammonium sulfate precipitate. Upon receipt, store at 2-8ºC in the dark, do not freeze.

Structural Characteristics
B-phycoerythrin is produced by red algae such as Rhodella sp. The protein is made up of at least three different subunits and varies according to the species of algae that produces it. The subunit structure of the most common B-PE is (αβ)6γ. The α subunit has two phycoerythrobilins (PEB), the β subunit has 3 PEBs and the γ subunit has 2 PEB and 2 phycourobilins (PUB). The molecular weight of B-PE is 240, 000 Daltons.
Excitation and emission profiles for SureLight™ B-PE. (Emission scan excitation wavelength at 540 nm.)
Compatible with:
532 nm – Diode-Pumped Solid State (DPSS) Laser
543 nm – He/Ne Laser
Spectral Characteristics
Absorption maximum -545 nm
Additional Absorption peak – 563.5
Emission maximum -572 nm
Extinction Coefficient (ε) – 2.41 x 106 M-1 cm-1
Quantum Yield (QY) – 0.98
Brightness (ε x QY) -2.36 x 106 M-1 cm-1
Specifications
A545/A280 ≥ 5.5
A620/A545 < 0.01
>95% single peak by HPLC
SureLight™ B-Phycoerythrin ( B-PE) Labeling Protocol:
Click here for a well-documented conjugation protocol - http://www.drmr.com/abcon/PE.html
REF: M. Roederer, Conjugation of monoclonal antibodies (August, 2004). http://www.drmr.com/abcon/