OF-02

OF-02 (OF-2) is an alkenyl amino alcohol (AAA) ionizable lipid for highly potent in vivo mRNA delivery.Alkenyl amino alcohols (AAA) are a functional group found in sphingosine and other bioactive molecules. It was used to prepare
AAA-based ionizable lipids through ring-opening reactions between alkenyl epoxides (AEs) and polyamine cores. These
AAA-based iLNPs could promote high-level protein expression Therefore, AAA-based ionizable lipids OF-00, OF-01,
OF-02, and OF-03 were prepared. The results of in vivo delivery
of human erythropoietin (hEPO) mRNA showed that the AAA
ionizable lipid OF-02 with the linoleic acid derivative
could effectively deliver hEPO mRNA. Compared with the positive
control CKK-E12, OF-02 showed an increased ability to
induce serum EPO protein expression by nearly twofold
(Figure 7b). Likewise, it outperformed two benchmark ionizable
lipids (503013 and C12-200) in the nucleic acid delivery field.
Furthermore, the mRNA delivered by OF-02 iLNPs was mainly
in vivo.translated into the liver. The liver-targeting ability of OF-02 iLNPs
improves their delivery efficiency. Therefore, the OF-02 iLNPs
may become excellent delivery vehicles for the treatment of liver
diseases without other side effects of damage to other organs
during the treatment