1.0 M HEPES

100 mL bottle of 1.0 M HEPES Optimize Reagent.
Synonyms: 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid or 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid or N-(2-Hydroxyethyl)piperazine-N'-(2-ethanesulfonic acid)
C8H18N2O4S
Mr 238.31
CAS Number [7365-45-9]
EC Number 230-907-9
Beilstein Registry Number 883043
Merck 14, 4654
RTECS TL6809000
MDL Number MFCD00006158
PubChem Substance ID 24895616
Purity > 99.5%
pKa 7.5 at 25 degrees Celsius
Useful pH range of HEPES buffer 6.8 - 8.2
Titrate HR2-585 to the desired pH between 6.8 - 8.2 using NaOH
HR2-585 Measured pH Range: 5.4 - 5.5 at 25 degrees Celsius
HR2-585 Measured Conductivity Range: 160.1 - 236.9 uS/cm at 25 degrees Celsius
HR2-585 Measured Refractive Index Range: 1.37093 - 1.37131 at 20 degrees Celsius
HR2-785 is titrated to pH 7.0 using NaOH
HR2-785 Measured Conductivity Range: 5.8 - 7.0 mS/cm at 25 degrees Celsius
HR2-785 Measured Refractive Index Range: 1.37133 - 1.37176 at 20 degrees Celsius
HR2-729 is titrated to pH 7.5 using NaOH
HR2-729 Measured Conductivity Range: 11.6 - 13.6 mS/cm at 25 degrees Celsius
HR2-729 Measured Refractive Index Range: 1.37177 - 1.37290 at 20 degrees Celsius
Insoluble Matter 0.01% max
DNase None Detected
RNase None Detected
Protease None Detected
Heavy Metals 1 ppm max
As 5 ppm max
Cu 5 ppm max
Fe 5 ppm max
Pb 5 ppm max
SO4 0.005% max