IL-4

3565

The ED50 as determined by the dose-dependent stimulation of human TF-1 cells is 0.1-0.5ng/ml.

IL-4 is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. Produced by mast cells, T cells and bone marrow stromal cells, IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergy. Recombinant human IL-4 is a 14.9 kDa globular protein containing 130 amino acid residues

130

NM_000589

NP_000580

> 98% by SDS-PAGE & visualized by silver stain

The lyophilized IL-4, though stable at room temperature, is best stored desiccated below 0 °C. Reconstituted IL-4 should be stored in working aliquots at -20 °C. Avoid repeated freeze-thaw cycles.

P05112