resDetect™ Universal Protein A Quick ELISA Kit (Boiling-free)

The kit is developed for the detection of natural or structurally conserved recombinant forms of Protein A and a recombinant form of Protein A with very significant structural differences from natural Protein A such as MabSelect SuReTM in Bioprocess manufacturing applications. It is used as a tool to aid in optimal purification process development and in routine quality control of in-process streams as well as final product.

Blue ice

The resDetect™ Universal Protein A Quick ELISA Kit (Boiling-free) is used to measure the levels of protein A and protein A variants by employing a standard sandwich-ELISA format. The micro-plate in the kit has been pre-coated with anti-protein A polyclonal antibody. Firstly, the standard samples provided in kit and your samples are treated with Denaturation Buffer to dissociation of protein A and antibody, stand a few minute. Before adding standards and samples, add the Biotin-Anti-Protein A Antibody to the plate to ensure that the standard samples are neutralized by the Biotin-Anti-Protein A Antibody buffer solution and protect the pre-coated antibody on the plate. Then, add the standard samples and your samples to the plate and form Antibody-antigen (Protein A) - biotinylated antibody complex, incubate and wash the wells. Next add Horseradish peroxidase conjugated streptavidin (Streptavidin-HRP) to the plate, incubate and wash the wells to remove any unbound reactants. At last, load the tetramethylbenzidine (TMB) substrate into the wells and monitor a blue color. The reaction is stopped by the addition of a stop solution and the color turns yellow. The intensity of the absorbance can be measured at 450nm and 630nm on a microtiter plate reader. The OD Value reflects the amount of protein A.

resDetect™ Universal Protein A Quick ELISA Kit (Boiling-free)

protein A (SuRe)