Neurofilament H (Neurofilament Heavy Polypeptide, NF-H, Neurofilament Triplet H Protein, 200kD Neurofilament Protein, Nefh, Nfh)

Antibodies / Antibodies-Neurofilament

-20°C

Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.

Neurofilament H

Antibodies to the various neurofilament subunits are very useful cell type markers since the proteins are among the most abundant of the nervous system, are expressed only in neurons and are biochemically very stable. Neurofilaments (NFs) are a type of intermediate filaments (IF) expressed
almost exclusively in neuronal cells, and in those cells most prominently in large axons. NFs in most vertebrates are composed of three different polypeptide chains with different molecular weights –neurofilament heavy protein (NF-H), medium (NF-M) and light protein (NF-L), which share sequence and structural similarity in a coiled-coil core domain, but differ in the length and sequence of their Ntermini and more dramatically of their C-termini which in the case of NF-M and NF-H form the flexible extensions that link NFs to each other and to other elements in the cytoplasm.

Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: Frozen and paraffin
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.