Microtubule Associated Protein 2 (MAP2, MAP-2)

Antibodies / Antibodies-Microtubule-associated Proteins

Blue Ice

Affinity Purified

30021010

Microtubules are 25nm diameter protein rods found in most kinds of eukarytic cells. They are polymerized from a dimeric subunit made of one a subunit and one b tubulin subunit. Microtubules are associated with a family of proteins called microtubule associated proteins (MAPs), which includes the protein t (tau) and a group of proteins referred to as MAP1, MAP2, MAP3, MAP4 and MAP5 (2). MAP2 is made up of two ~280kD apparent molecular weight bands referred to as MAP2a and MAP2b. A third lower molecular weight form, usually called MAP2c, corresponds to a pair of protein bands running at ~70kD on SDS-PAGE gels, and is expressed early in development. All these MAP2 forms are derived from a single gene by alternate transcription, and all share a C-terminal sequence which includes either three or four microtubule binding peptide sequences, which are very similar to those found in the related microtubule binding protein t (tau). MAP2 isoforms are expressed only in neuronal cells and specifically in the perikarya and dendrites of these cells. Antibodies to MAP2 are therefore excellent markers on neuronal cells, their perikarya and neuronal dendrites. In contrast t (tau) is found predominantly in neuronal axons. The HGNC name for this protein is MAP2.

Applications:
Suitable for use in Immunofluorescence, Immunocytochemistry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IC): 1:1000
Western Blot: 1:10, 000 using chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.