PARK7 (CAP1, Oncogene DJ1, Parkinson Disease (Autosomal Recessive Early Onset) 7, RNA Binding Protein Regulatory Subunit, SP22)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a liquid in PBS, 10mM sodium azide.

Full length recombinant human DJ-1 expressed in and purified from E. coli.

DJ-1, also known as Park7, is a cytoplasmic protein that belongs to the ThiJ/Pfp1/DJ1 superfamily that function as protein chaperones, catalases, proteases and kinases. DJ-1 was originally cloned as an oncogene that cooperatively transforms cells together with H-ras (1). Mutations in the DJ-1 gene are associated with rare forms of autosomal recessive early-onset Parkinson's disease (2). DJ-1 may also function as a redox-sensitive chaperone, as a sensor for oxidative stress, and it apparently protects neurons against oxidative stress and cell death (3-5). Augmenting DJ-1 activity might provide novel approaches to treating chronic neurodegenerative illnesses such as Parkinson's disease and acute damage such as stroke (6). The HGNC name for this protein is PARK7.

Applications:
Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. On blots 031417 reveals a prominent ~21kD band, and on cells in tissue culture the antibody stains mainly in cytoplasm. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:5000
Western Blot: 1:5000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.