MAP2a,b,c (BSA & Azide Free) (Microtubule Associated Protein 2a,b,c)

Mab

Bovine, Human, Rat, Xenopus

-20°C

Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M2350-01B.

Bovine brain microtubule associated protein 2. Epitope: aa1-151. Cellular Localization: Cytoplasmic.

The expression of MAP2 is developmentally regulated and its multiple forms arise by alternative splicing of a single gene.

Applications:
Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
IMR-5 or T98G human glioblastoma cells or brain

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.