Tyrosinase (TYR, LB24 AB, LB24-AB, Monophenol Monooxygenase, Oculocutaneous Albinism IA, OCA1A, OCAIA, SK29 AB, SK29-AB, Tumor Rejection Antigen AB) (BSA & Azide Free)

Antibodies / Antibodies-Disease Markers

-20°C

Supplied as a liquid in PBS, pH 7.4. No preservatives or stabilizing proteins added. Also available with BSA and azide. See T9230-05.

Recombinant tyrosinase protein. Epitope: 1-433. Cellular Localization: Cytoplasmic

Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3, 4-dihydroxy- phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with antibody showed tyrosinase in melanotic as well as amelanotic variants (1). This antibody is a useful marker for melanocytes and melanomas.

Applications:
Suitable for use in Immunocytochemistry, Western Blot, Immunofluorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:25-1:100 for 2 hours at RT.
Immunohistochemistry (formalin/paraffin): 1:50-1:100 for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 8.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Melanoma cell lines and Melanoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.