TLR2 (Toll Like Receptor 2, CD282) (FITC)

Antibodies / Antibodies-Toll Like Receptors (TLR)

-20°C

Supplied as a liquid in PBS, 1% BSA, 0.02% sodium azide. Labeled with fluorescein isothiocyanate (FITC).

Recombinant protein corresponding to human TLR2 expressed in the CHO/CD14 reporter cell line.

Toll-like receptors (TLR) are highly conserved throughout evolution and have been implicated in the innate defense to many pathogens. In mammals, TLR identified as type I transmembrane signaling receptors with pattern recognition capabilities, have been implicated in the innate host defense to pathogens. T8050-09V is a TLR2 function blocking antibody that is useful for studies on the role of TLR2 as a pattern recognition receptor in microbial products induced cytokine production by TLR2 bearing cells such as human PBMCs.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence, Functional Studies and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml showed a band at ~90kD in non-reduced peripheral blood mononuclear cells.
Immunohistochemistry (frozen): 1:50 for acetone fixed tissues using 3% H2O2 for blockade of endogenous peroxidases
Immunohistochemistry (paraffin): 1ug/ml for HOPE-fixed tissue for 16hr at 4°C.
Immunoprecipitation: T8050-09V conjugated Sepharose immunoprecipitated lysed monocytes
Immunofluorescence: 5-10ug for staining HMECs fixed with 4% paraformaldehyde in PBS, permeabilized and blocked.
Functional Studies: 1ug/5x10e7 blocks TNF-a release induced by bacteria from periperal blood mononuclear cells
Flow Cytometry: 2ug/3x10e5 untreated or 0.4% formaldehyde fixed cells. Permeabilized or 4% paraformaldehyde fixed cells are not recommended for this application.
Optimal dilutions to be determined by the researcher.

Positive Control:
Peripheral blood mononuclear cells (PBMCs), granulocytes and monocytes.

Storage and Stability:
Store product at 4°C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C. Aliquots are stable at -20°C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.