MCAM (Melanoma Cell Adhesion Molecule) (PE)

Mab

IgG2b, k

4°C Do Not Freeze

Supplied as a liquid in PBS, 0.09% sodium azide and a stabilizing agent. Labeled with R-Phycoerythrin (PE).

Pooled Ed15 and Ed16 thymocytes from H.B15, H.B19 and H.B21 chicken embryos

Chicken MCAM, a member of the immunoglobulin superfamily of cell surface receptors, consists of the V-V-C2-C2-C2 Ig domains. It is a single chain glycoprotein with three mRNA splice variants. One has a short cytoplasmic tail, another has a long tail, and the third seems to lack transmembrane and cytoplasmic regions. It is highly homologous to gicerin, a molecule involved in neurite outgrowth and Wilm’s kidney tumor progression in the chicken, and to the human melanoma progression molecule MUC18. Chicken MCAM is expressed by c-kit+ hematopoietic progenitor cells in embryonic bone marrow, thymocytes, and capillary endothelial cells. MCAM-expressing cells sorted from bone marrow are enriched in progenitors capable of differentiating into T cells when transferred into the appropriate thymic microenvironment. MCAM has been proposed to be involved in cellular adhesion and homing processes.1

Applications:
Suitable for use in Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: ≤0.2ug/10e6 cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.