Ovalbumin (HRP)

Antibodies / Antibodies-Albumin

-20°C

Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease free), 0.01% gentamicin sulfate. Reconstitute with 100ul sterile ddH2O. Labeled with Horseradish peroxidase (HRP)

Ovalbumin (Hen Egg White)

Ovalbumin is a glycoprotein and the major protein of egg white. It has a molecular weight of 43, 000. Ovalbumin is used for conjugation or immunological determinations and drug and pharmaceutical processing.

Applications:
Suitable for use in Western Blot, ELISA, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1; 1000-1:5000
ELISA: 1:5000-1:25, 000
Immunoprecipitation: 1:100
Immunohistochemistry: 1:500-1:2500
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.