Glucose Regulated Protein 78 (GRP78, GRP-78, 78kD Glucose-regulated Protein, Endoplasmic Reticulum Lumenal Ca(2+)-binding Protein grp78, Heat Shock 70kD Protein 5, HSPA5, Immunoglobulin Heavy Chain-binding Protein, BiP)

Antibodies / Antibodies-Heat Shock Proteins

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol.

Full length human GRP78 (Bip) at the N-terminal, fused to His-tag.

GRP78 is a ubiquitously expressed, 78kD glucose- regulated protein, and is commonly referred to as an immunoglobin chain binding protein (BiP). The BiP proteins are categorized as stress response proteins because they play an important role in the proper folding and assembly of nascent protein and in the scavenging of misfolded proteins in the endoplasmic reticulum lumen. Translation of BiP is directed by an internal ribosomal entry site (IRES) in the 5' nontranslated region of the BiP mRNA. BiP IRES activity increases when cells are heat stressed (1). GRP78 is also critical for maintenance of cell homeostasis and the prevention of apoptosis (2). Luo et al. have provided findings that suggest GRP78 is essential for embryonic cell growth and pluripotent cell survival (3). In terms of diseases, GRP78 has been shown to be a reliable biomarker of hypoglycemia, to serve a neuroprotective function in neurons exposed to glutamate and oxidative stress (4), and its protein levels are reduced in the brains of Alzheimer’s patients (5). Also, the induction of the GRP78 protein that results in severe glucose and oxygen deprivation could possible lead to drug resistance to anti-tumor drugs (6, 7).

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot (ECL): 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.