SMAD2,3 (Mothers Against Decapentaplegic Homolog 2,MAD Homolog 2,Mothers Against DPP Homolog 2,JV18-1,Mad-related Protein 2,hMAD-2,SMAD Family Member 2,SMAD 2,MADH2,MADR2/Mothers Against Decapentaplegic Homolog 3,MAD Hom

Antibodies / Antibodies-Smad Proteins

-20°C

Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.

Synthetic peptide corresponding to residues surrounding Ala208 of human Smad2. Species sequence homology: Xenopus (100%)

Members of the Smad family of signal transduction molecules are components of a critical intracellular pathway that transmit TGF-b signals from the cell surface into the nucleus. Three distinct classes of Smads have been defined: the receptor-regulated Smads (R-Smads), which include Smad1, 2, 3, 5, and 8; the common-mediator Smad (co-Smad), Smad4; and the antagonistic or inhibitory Smads (I-Smads), Smad6 and 7. Activated type I receptors associate with specific R-Smads and phosphorylate them on a conserved carboxy-terminal SSXS motif. The phosphorylated R-Smad dissociates from the receptor and forms a heteromeric complex with the co-Smad (Smad4), allowing translocation of the complex to the nucleus. Once in the nucleus, Smads can target a variety of DNA binding proteins to regulate transcriptional responses.

Applications:
Suitable for use in Immunofluorescence/ Immunocytochemistry, Flow Cytometry, Western Blot, Immunoprecipitation, Chromatin IP, Chip Aragose and Chip Magnetic. Other applications not tested.

Recommended Dilution:
Immunofluorescence (IF-IC): 1:125
Flow Cytometry: 1:200
Western Blot: 1:1000
Immunoprecipitation: 1:50
Chromatin IP: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.