c-Fos (Fos)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.

Synthetic peptide corresponding to human FOS at N-Terminal.

C-Fos is the product of an immediate early gene. It is a major nuclear target for signal transduction pathways involved in the regulation of cell growth, differentiation, and transformation. The Fos and Jun proteins from dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. Studies in rodents have shown that c-Fos is essential in regulating neuronal cell survival versus death. c-Fos is induced by neuronal activity, regulates the expression of the kainic acid receptor GLUR6 and brain derived neurotrophic factor (BDNF).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:10, 000
Western Blot: 1:500-1:1000
Immunohistochemistry (Paraffin): 10ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.