Caspase 3 (caspase 3, apoptosis-related cysteine peptidase, apopain,CASP-3, Caspase-3, CPP32, CPP-32, CPP32B, Cysteine Protease CPP32, SCA-1, SREBP cleavage activity 1, Yama, Yama Protein, CASP3)

Antibodies / Antibodies-Apoptosis, Caspase

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4, 5% BSA, 0.05% thimerosal, 0.05% sodium azide. Reconstitute with sterile dH2O.

Synthetic peptide corresponding to a sequence at the C-terminal of human Caspase-3(P10), identical to the related rat and mouse sequence.

Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32kD) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17kD) and small (12kD) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot:1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.