Fragile X Mental Retardation Protein 1 (FMR1, FMR-1, FMRP, FRAXA, MGC87458, POF, POF1, Protein FMR-1)

Antibodies / Antibodies-RNA Binding Proteins

-20°C

Supplied as a liquid. No preservatives added.

Fusion protein with a full length human FMRP.

Fragile X Mental Retardation Protein (FMRP) is an RNA-Binding protein that is associated to polysomes and may be involved in the transpot of mRNA from the nucleus to the cytoplasm. Defects in FMR1 are the cause of Fragile X syndrome, which is a common genetic disease characterized by moderate to severe mental retardation, macroorchidism, large ears, prominent jaw, and high-pitched, jocular speech. The defect in most fragile X syndrome patients results from an amplication of a CGG repeat regions with is directly in front of the coding region.

Applications:
Suitable for use in Western Blot, ELISA, Immunohistochemistry, Immunocytochemistry and the detection of FMRP on blood smears. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:500 detected FMRP on 10ug of mouse E17 spinal cord lysate. Lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with F6072. Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
ELISA: 1:500-1:5000
Immunohistochemistry (FFPE): 1:500-1:5000
Immunocytochemistry: 1:500-1:5000 was used on transfected cells. Light fixation (2% PFA, permeabilize with 0.1% Triton in block only)
Detection of FMRP on Blood Smears: 1:500-1:5000
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa whole cell lysate, HeLa nuclear lysate, mouse E17 spinal cord lysate

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.