Glucose Regulated Protein 94 (Grp94, gp96)

Antibodies / Antibodies-Heat Shock Proteins

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.

Synthetic peptide corresponding to the N-terminal region of the mouse and human gp96 protein. Species Sequence Homology: porcine, bovine, and canine; 100%

The tumor rejection antigen (gp96), also known as glucose-regulated protein 94 (GRP94), is a member of the heat shock protein (HSP) family. The gp96 protein is up-regulated by stress conditions such as glucose starvation and heat shock, and its function is regulated through a ligand-dependent conversion from an active to an inactive conformation. gp96 is involved in both innate and adaptive immunity. A study has shown that a B-cell line deficient in gp96 is unresponsive to microbial stimuli because toll-like receptors (TLRs) are retained intracellularly. However, normal surface levels of TLRs were restored when these cells were transfected with a cDNA encoding gp96. The tumor rejection antigen can activate the release of nitric oxide by antigen presenting cells (APCs) and it also has a relevant role in the regulation of immune responses to pathological cell death.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
ELISA: 0.1-1ug/ml
Western Blot: 1-3ug/ml. Identifies a single band at ~96kD
Immunoprecipitation: 1-5ug/ml
Immunohistochemistry: 0.5-1ug/ml Heat induced epitope retrieval (HIER) with EDTA, pH 8.0, is required prior to staining for formalin-fixed, paraffin-embedded (FFPE) tissues.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.