Glutathione Peroxidase 4 (GPX4 (phospholipid hydroperoxidase) GPX-4, MCSP, PHGPx, Phospholipid hydroperoxide glutathione peroxidase, mitochondrial, snGPx, snPHGPx)

Antibodies / Antibodies-Enzymes, Peroxidase

-20°C

Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.

E. coli-derived rhGPX4.

Glutathione Peroxidase 4 (GPX4; also known as PHGPx) is a monomeric, 21kD member of the glutathione peroxidase family of proteins. It is widely expressed and serves to both protect cell membranes from phospholipid and cholesterol hydroperoxidases, and form an inactive enzyme structural component of the sperm mitochondrial capsule. Human GPX4 is
197 amino acids (aa) in length and contains a mitochondrial targeting sequence (aa 1-27) and an enzymatically active selenocysteine at Sec73. During sperm maturation, the targeting sequence is cleaved, and GPX4 may form inactive covalently-linked oligomers. There are multiple splice variants. One is ubiquitously expressed, cytoplasmic, and shows
an alternate start site at Met28. A second is 226 aa in length, nuclear, and shows an Arg-rich 65 aa substitution for aa 1-28. Over amino acids 74-168, human GPX4 shares 93% aa identity with mouse GPX4.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.5ug/ml using lysates of mouse, human, and rat liver tissue
Optimal dilution determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.