MAP Kinase p44/42 (MAPK3/MAPK1,Mitogen-activated Protein Kinase 1,MAP Kinase 1,MAPK 1,Mapk,ERT1,Extracellular Signal-regulated Kinase 2,ERK-2,MAP Kinase Isoform p42,p42-MAPK,Mitogen-activated Protein Kinase 2,MAP Kinase

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

Synthetic peptide (a.a. 339–353) corresponding to a C-terminal segment of rat ERK1.

Mitogen activated protein kinases (MAPKs) play pivotal roles in mediating signal transduction from the cell surface to the nucleus (1) . These kinases are encoded by distinct genes and together form a family of kinases whose activation is dependent upon dual phosphorylation on specific threonine and tyrosine residues. In yeast, a number of different MAP Kinases have been identified and are activated by distinct signaling pathways

Applications:
Suitable for use in ELISA, Western Blotting, Immunoprecipitation, Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 0.1–1ug/ml (to coat: 10ug/ml)
Immunoprecipitation (10) : 2–5ug
Immunohistochemistry: 5–10ug/ml
Western blotting (9–11) : ~1:1000

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.