Neprilysin (Atriopeptidase, Common Acute Lymphocytic Leukemia Antigen, CALLA, Enkephalinase, Neutral Endopeptidase 24.11, NEP, Neutral Endopeptidase, CD10, MME, EPN)

Antibodies / Antibodies-Neuroscience

-20°C

Supplied as a lyophilized powder from PBS, 0.05% sodium azide.

Recombinant corresponding to human Nep protein, EC domain.

The amyloid b-peptide (Ab) of 39-43aa is constitutively produced in brain upon proteolysis of the b-amyloid precursor protein (APP) and exists as fragments of 40, 42 and 43aa (Ab40, Ab42, Ab43). In the young and healthy humans, the secreted Ab is rapidly catabolized before it can be deposited in the brain. However, upon aging or the onset of familial Alzheimer's disease, alterations in either synthesis or degradation/clearance of Ab may contribute to amyloid depositions in the brain. Ab carries cleavage sites for a number of in vivo and in vitro proteases like cathepsin D and M-13 metalloproteases. The M-13 family comprises several zinc-dependent metalloproteases like DINE, PHEX, KELL, ECE, XCE, neprilysin (NEP) and neprilysin-like proteases (NEPL-a, NEPL-b, NEPL-g).

NEP [variously termed as neutral endopeptidase-24.11 (NEP), neprilysin, enkephalinase, EC3.4.24.11, common acute lymphoblastic leukemia antigen (CALLA), CD10] is the key in vivo enzyme degrading biopeptides Ab, substance-P and enkephalin in brain; atrial natriuretic peptide, bradykinin and endothelin-1 in kidney. It is a 97kD ectoenzyme (mouse, rat, human 750aa) with a large extracellular domain containing its catalytic site, which can degrade Ab on cell surface. NEP is ubiquitous but its high expression in brain is restricted to striatum, olfactory tubercle, substantia nigra, choroids plexes, endopeduncular nucleus, pontine nucleus, and cerebellum and in many peripheral tissues, particularly in brush-border membranes. The poor expression of NEP in the hippocampus and cerebral cortex is reflected in the selective deposition of Ab42 in these regions. NEP is also expressed in soluble form in human plasma and cerebrospinal fluid. NEP can degrade both synthetic and cell secreted Ab40 and Ab42 and may be a good therapeutic target for the treatment of Alzheimer's disease.

Applications:
Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested.

Recommended Dilution:
Western Blot: 1-5ug/ml
ELISA: 1:5000-1:50, 000 (using 50-100ng control peptide/well)
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O or PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.