Stat3, phosphorylated (Ser727) (Signal Transducer and Activator of Transcription 3, Acute-phase Response Factor, APRF)

Antibodies / Antibodies-Transcription Factors, STAT

-20°C

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol. No preservative added.

Synthetic phosphopeptide (KLH coupled) corresponding to residues around Ser727 of mouse Stat3.

Stat3 is a key signaling molecule for many cytokines and growth-factor receptors (1) and is required for murine fetal development (2). Additionally, Stat3 is constitutively activated in a number of human tumors (3, 4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by tyrosine phosphorylation at Tyr705, which induces dimerization, nuclear translocation and DNA binding (6, 7). Transcriptional activation seems to be regulated by serine phosphorylation at Ser727, apparently via the MAPK or mTOR pathways (8, 9). Stat3 isoform expression appears to reflect biological function: the relative expression levels of Stat3alpha (86kD) and Stat3beta (79kD) depend on cell type, ligand exposure or maturation stage of the cells (10). It is notable that Stat3beta lacks the serine phosphorylation site within the C-terminal transcriptional activation domain (8).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunofluorescence (IC). Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunofluorescence (IC) 1:100. Methanol permeabilization required.
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.