VAMP 2 (Vesicle Associated Membrane Protein 2, Vesicle-associated Membrane Protein 2, VAMP2, VAMP-2, Synaptobrevin 2, Synaptobrevin-2, SYB 2, SYB2, SYB-2)

Antibodies / Antibodies-Membrane Proteins

-20°C

Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with sterile PBS.

Purified, E.coli-derived, recombinant human VAMP-2 (aa1-94; Accession # P63027).

VAMP-2 (vesicle associated membrane protein 2; also synaptobrevin-2) is a 13kD member of the synaptobrevin family of proteins. It is a type IV transmembrane (TM) protein (i.e.- a type II TM protein whose C-terminus is almost completely transmembrane) that is found in the presynaptic terminals of neurons. VAMP-2 is targeted to presynaptic vesicles following binding to synaptophysin I. Dissociation allows for synaptic vesicle fusion at the synaptic cleft with subsequent granule release. Human VAMP-2 is 116aa in length. It contains one acetylation site at Ser2, a vSNARE coiled-coil homology region (aa31-91) and a membrane-anchor domain (aa95-114). Overaa1-94, human VAMP-2 shares 100% and 99% aa identity with canine and mouse VAMP-2, respectively.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 0.5-1ug/ml
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.