Erk1/2,diphosphorylated (Extracellular Signal-regulated Kinase 1,ERK1,ERK-1,ERK,Extracellular Signal-regulated Kinase 2,ERK2,ERK-2,ERT1,ERT2,Insulin-stimulated MAP2 Kinase,MAP Kinase Isoform p42,MAP Kinase Isoform p44,Mi

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a lyophilized powder from 1.2% sodium acetate, 2mg BSA, 0.01mg sodium azide. Reconstitute with 1.2% sodium acetate, PBS.

Human activated Map kinase.

Extracellular signal regulated kinase (ERK), also known as mitogen activated protein kinase (MAPK), is a family with two homologous members; ERK1 (44kD) and ERK2 (42kD). This antibody reacts with the active forms of these proteins that have specific phosphorylated Threonine and Tyrosine residues within a Thr-Glu-Tyr motif (Thr202/Tyr204 for ERK1 and Thr185/Tyr187 for ERK2).

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-0.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with 1.2% sodium acetate. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.