Nestin, CT (NES)

Antibodies / Antibodies-Proteins

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Nestin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1560ca.1590 amino acids from the C-terminal region of human nestin.

Nestin is a class VI intermediate filament protein expressed predominantly in stem cells of the neural tube but absent from virtually all differentiated CNS cells. In the CNS, nestin is downregulated upon differentiation and replaced by neurofilaments. Transient expression of nestin has been postulated as a key step committing cells to the neural differentiation pathway. Nestin expression has also been observed in pancreatic hematopoietic stem cell populations.

Applications:
Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500
Immunofluorescence: 1:10-50
Immunohistochemistry: 1:50-100

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.