PRPS1, PRPS2, PRPS3, NT (Ribose-phosphate pyrophosphokinase 1, PPRibP, Phosphoribosyl pyrophosphate synthase I)

Antibodies / Antibodies-Enzymes, Synthase

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

PRPS1/PRPS2/PRPS3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87ca.116 amino acids from the N-terminal region of human PRPS1/2/3.

Phosphoribosylpyrophosphate synthetase (PRPS; EC 2.7.6.1) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine, pyrimidine, and pyridine biosynthesis. By PCR of human lymphoblast mRNA using primers based on the cDNA sequence of rat PRS I (Prps1), Roessler et al. (1990) isolated a partial human PRPS1 cDNA. They used this partial cDNA to screen lymphoblast cDNA libraries and isolated additional cDNAs corresponding to the entire PRPS1 coding region. The deduced PRPS1 protein has 318 amino acids.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.