CTAGE1 (Cutaneous T-cell Lymphoma-associated Antigen 1, Protein cTAGE-1, Cancer/Testis Antigen 21.1, CT21.1, CTAGE2, Cutaneous Lymphocyte Antigen, CLA)

Mab

IgM, k

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

Human Cutaneous Lymphocyte Antigen.

Cutaneous Lymphocyte-assiciated Antigen (CLA) is a 140kD homodimer protein recognized by a unique monoclonal antibody. It is expressed on T lymphocytes in skin, subsets of peripheral blood memory T cells, NK cells, memory B cells and dendritic cells, as well as on monocytes, granulocytes and activated endothelial cells. CLA is a carbohydrate epitope of sialic acid- and fucose-modified P-selectin glycoprotein ligand-1 (PSGL-1), a surface glycoprotein expressed on majority of peripheral blood leukocytes. CLA is a subset ligand for E-selectin, P-selectin, and L-selectin. It plays a role in memory lymphocyte homing, tethering and rolling. Treatment of activated HUVEC cells with this antibody inhibits lymphocyte adhesion.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: ≤2ug/10e6 cells in 100ul
Immunohistochemistry: Acetone-fixed, frozen and formalin fixed paraffin embedded sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.