Frizzled 4 (Frizzled-4,Frizzled (Drosophila) Homolog 4,Fz 4,Fz-4,FZD4,FZD4S,FzE4,CD344 Antigen,EVR1,FEVR,GPCR,hFz4,MGC34390,WNT Receptor Frizzled-4,Exudative Vitreoretinopathy 1 Autosomal Dominant Criswick-Schepens Syndr

Antibodies / Antibodies-Disease Markers

-20°C

Supplied as a lyophilized powder in PBS, pH 7.2, 0.5% BSA. Labeled with Biotin. Reconstitute with 1ml sterile PBS.

NS0-derived rmFrizzled-4 cysteine-rich domain.

Frizzled-4 belongs to the Frizzled family of 7-pass transmembrane proteins and binds to Wnt-8. It is implicated in several developmental processes, notably in retinal angiogenesis.

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry and Western Blot. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 0.25ug labels 10e6 cells using HEK293 human embryonic kidney cell line
Immunocytochemistry: 8-25ug/ml using immersion fixed HEK293 human embryonic kidney cell line
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.