Toll Like Receptor 2, NT (TLR2, CD282)

Antibodies / Antibodies-Receptors

-20°C

Supplied as a liquid in PBS, 0.02% sodium azide.

Peptide corresponding to an 14 amino acid sequence near the amino terminus of human TLR2.

Toll Like Receptor 2 (TLR2) is a 90kD type 1 transmembrane protein that is primarily expressed by peripheral blood monocytes. TLR2 is also expressed in the spleen, bone marrow and lymph nodes. TLR2 is reported to respond to a diverse range of bacterial cell wall components, mediating the innate immune response in co-operation with MD-2.

Applications:
Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml; Detected a band of ~100kD in A-20 cell lysates.
Immunohistochemistry (Paraffin): 2ug/ml. Requires antigen retrieval using heat treatment prior to staining paraffin sections. Sodium citrate buffer, pH 6.0 is recommended.
Immunofluorescence: 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human spleen

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.