TLR8 (Toll Like Receptor 8, Toll-like Receptor 8, TLR 8, CD288, CD288 Antigen, MGC119599, MGC119600)

Antibodies / Antibodies-Toll Like Receptors (TLR)

-20°C

Supplied as a liquid in PBS, pH 7.4, 0.05% sodium azide.

Synthetic peptide corresponding to aa 750-850 of human TLR8 (KLH coupled).

The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules (1). The TRL family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition (2). Ten human homologs of TLRs (TLR1-10) have been described (3). TLR8 gene contains three exons, two of which have coding function (4). TLR8 cDNA codes for a protein of approximate molecular weight of 120kD (4, 5).

Applications:
Suitable for use in Flow Cytometry, Immunohistochemistry, Western Blot, Immunocytochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1-3ug/ml. Cell lysate from 293 transfected with human TLR8 was used as positive control. A protein of~20kD was detected.
Flow Cytometry (intracellular): 2-5ug/1x10e6 cells. Ramos cells can be used as a positive control.
Immunohistochemistry (paraffin): 5ug/ml. Requires HIER using sodium citrate buffer pH6.0 prior to staining.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.