c-Fos (Fos)

Antibodies / Antibodies-Oncoproteins

-20°C

Supplied as a lyophilized powder. No preservative added. Reconstitute with 500ul sterile ddH2O.

Synthetic c-fos peptide corresponding to aa4-17, Ser-Gly-Phe-Asn-Ala Asp-Tyr-Glu-Ala-Ser-Ser-Ser-Arg-Cys of human Fos, conjugated to bovine thyroglobulin. Species Sequence Homology: mouse

c-fos is a member of a family of immediate early gene (IEG) transcription factors (other members include Jun and Egr-1) also identified as proto-oncogenes. The basal expression of c-fos and other IEGs is typically low but increases relatively quickly and often dramatically (15-30 min for mRNA; 30-60 min for protein) in response to changes in cellular activity typically caused by external stimuli, such as stress or neuronal activation. Thus, these IEGs are being used to indicate specific activity driven cellular activation including the identification of neurons activated by various growth factors, neuroactive drugs, and correlated changes in behavioral or physiological states.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 10ug/ml Detects a band at ~55kD.
Immunohistochemistry: 1:500-1:4000 (4°C, 1-3 days) Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase. Recommended fixation is 4% PFA with 0.5% picric acid (Li et al 1999). Triton X-100 pretreatment in the block only. Antigen retrieval for paraffin sections is recommended. Microwave citric acid treatment suggested.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.