Tubulin, beta 3 (Tubulin beta III, Tubulin beta 3 Chain, TUBB 3, TUBB3, Neuron Specific beta III Tubulin, beta-4, MC1R, Tubulin beta 4, TUBB 4, TUBB4)

Antibodies / Antibodies-Tubulin

-20°C

Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS.

Rat brain-derived microtubules

b-III Tubulin, also known as tubulin b-4, is regarded as a neuron-specific marker. The expression of b-III Tubulin has been suggested to be one of the earliest markers to signal commitment in primitive neuroepithelium.

Applications:
Suitable for use in Immunocytochemistry, Immunofluorescence and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 8-25ug/ml with immunofluorescence detection. Used in immersion-fixed rat cortical stem cells and immersion-fixed rat cortical neurons.
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.