GFAP (Glial Fibrillary Acidic Protein)

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as a liquid, 0.03% sodium azide.

Recombinant protein corresponding to aa292-432 from human GFAP, expressed in E. coli.

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.Tissue specificity: Expressed in cells lacking fibronectin.ABCAM:It is heavily, and specifically, expressed in astrocytes and certain other astroglia in the central nervous system, in satellite cells in peripheral ganglia, and in non myelinating Schwann cells in peripheral nerves.In addition many types of brain tumor, presumably derived from astrocytic cells, heavily express GFAP. GFAP is also found in the lens epithelium, Kupffer cells of the liver, in some cells in salivary tumors and has been reported in erythrocytes.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500-1:2000.
Immunohistochemistry: 1:200-1:1000.
Immunofluorescence: 1:200-1:1000.
ELISA: 1:10, 000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.