ADA, CT (ADA, ADA1, Adenosine deaminase, Adenosine aminohydrolase)

Antibodies / Antibodies-Enzymes

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 287-314 in the C-terminal region of human ADA, UniProt Accession #P00813.

This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine. Various mutations have been described for this gene and have been linked to human diseases. Deficiency in this enzyme causes a form of severe combined immunodeficiency disease (SCID), in which there is dysfunction of both B and T lymphocytes with impaired cellular immunity and decreased production of immunoglobulins, whereas elevated levels of this enzyme have been associated with congenital hemolytic anemia.

Applications:
Suitable for use in Western Blot, Indirect Flow Cytometry, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000
Flow Cytometry (Indirect): 1:25
Immunohistochemistry (Paraffin, Leica): 1; 500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.