PAPOLG, CT (PAPOLG, PAP2, PAPG, Poly(A) polymerase gamma, Neo-poly(A) polymerase, Polynucleotide adenylyltransferase gamma, SRP RNA 3'-adenylating enzyme)

Antibodies / Antibodies-Enzymes, Polymerase

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

PAPOLG antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 684-714 amino acids from the C-terminal region of human PAPOLG.

This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14.

Applications:
Suitable for use in Western Blot, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.