RAD17, ID (RAD17, R24L, Cell cycle checkpoint protein RAD17, RF-C/activator 1 homolog)

Antibodies / Antibodies-DNA Replication, Repair

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

RAD17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-254 amino acids from the Central region of human RAD17.

The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad17, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This protein shares strong similarity with DNA replication factor C (RFC), and can form a complex with RFCs. This protein binds to chromatin prior to DNA damage and is phosphorylated by the checkpoint kinase ATR following damage. This protein recruits the RAD1-RAD9-HUS1 checkpoint protein complex onto chromatin after DNA damage, which may be required for its phosphorylation. The phosphorylation of this protein is required for the DNA-damage-induced cell cycle G2 arrest, and is thought to be a critical early event during checkpoint signaling in DNA-damaged cells. Eight alternatively spliced transcript variants of this gene, which encode four distinct proteins, have been reported. Two pseudogenes, located on chromosomes 7 and 13, have been identified.

Applications:
Suitable for use in Western Blot, Indirect Flow Cytometry, ELISA

Recommended Dilution:
ELISA: 1:1, 000
Western Blot: 1:100-500
Indirect Flow Cytometry: 1:10-50

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.