SKA2, NT (SKA2, FAM33A, Spindle and kinetochore-associated protein 2, Protein FAM33A)

Antibodies / Antibodies-Mitosis Proteins

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide

SKA2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 16-45 amino acids from the N-terminal region of human SKA2.

Component of the SKA1 complex, a microtubule-binding subcomplex of the outer kinetochore that is essential for proper chromosome segregation. Required for timely anaphase onset during mitosis, when chromosomes undergo bipolar attachment on spindle microtubules leading to silencing of the spindle checkpoint. The SKA1 complex is a direct component of the kinetochore-microtubule interface and directly associates with microtubules as oligomeric assemblies. The complex facilitates the processive movement of microspheres along a microtubule in a depolymerization-coupled manner. In the complex, it is required for SKA1 localization.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.