TCRB, ID (TCRB, T-cell receptor beta chain V region CTL-L17)

Antibodies / Antibodies-Receptors

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

TCRB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 74-102 amino acids from the Central region of human TCRB.

The receptors on T cells consist of immunoglobulin like integral membrane glycoproteins containing 2 polypeptide subunits, alpha and beta, of similar molecular weight, 40 to 55 kD in the human. Like the immunoglobulins of the B cells, each T cell receptor subunit has, external to the cell membrane, an N terminal variable domain and a C terminal constant domain. T cell receptors recognise foreign antigens which have been processed as small peptides and bound to major histocompatibility complex molecules at the surface of antigen presenting cells. Each T cell receptor is a dimer consisting of one alpha and one beta chain or one delta and one gamma chain. In a single cell, the T cell receptor loci are rearranged and expressed in the order delta, gamma, beta, and alpha. If both delta and gamma rearrangements produce functional chains, the cell expresses delta and gamma. If not, the cell proceeds to rearrange the beta and alpha loci.

Applications:
Suitable for use in Western Blot, Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Flow Cytometry (Indirect): 1:25
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.