TNF,CT (TNF,TNFA,TNFSF2,Tumor necrosis factor,Cachectin,TNF-alpha,Tumor necrosis factor ligand superfamily member 2,Tumor necrosis factor,membrane form,N-terminal fragment,Intracellular domain 1,Intracellular domain 2,C-

Antibodies / Antibodies-Growth Factors, Cytokines

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide

TNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 186-216 amino acids from the C-terminal region of human TNF.

This gene encodes a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophages. It can bind to, and thus functions through its receptors TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. This cytokine is involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer. Knockout studies in mice also suggested the neuroprotective function of this cytokine.

Applications:
Suitable for use in Western Blot, Indirect Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:1000
Indirect Flow Cytometry: 1:10-1; 50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.