XRCC5,ID (K439) (XRCC5,G22P2,X-ray repair cross-complementing protein 5,86kD subunit of Ku antigen,ATP-dependent DNA helicase 2 subunit 2,ATP-dependent DNA helicase II 80kD subunit,CTC box-binding factor 85kD subunit,DNA

Antibodies / Antibodies-DNA Replication, Repair

-20°C

Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

XRCC5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 431-458 amino acids from the Central region of human XRCC5.

The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.

Applications:
Suitable for use in Western Blot, Immunofluorescence, Indirect Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunofluorescence: 1:10-1:50
Indirect Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.