ACK1, phosphorylated (Tyr518) (Tyrosine Kinase Non-receptor Protein 2, Activated CDC42 Kinase 1, ACK-1, TNK2)

Antibodies / Antibodies-Protein Kinases

-20°C

Supplied as a liquid in PBS, 0.09% sodium azide.

Synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr518 of human ACK1 (KLH).

ACK1 is a tyrosine kinase that binds Cdc42Hs in its GTP-bound form and inhibits both the intrinsic and GTPase-activating protein (GAP)-stimulated GTPase activity of Cdc42Hs. This binding is mediated by a unique sequence of 47aa C-terminal to an SH3 domain. The protein may be involved in a regulatory mechanism that sustains the GTP-bound active form of Cdc42Hs and which is directly linked to a tyrosine phosphorylation signal transduction pathway.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1, 000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.