Angiotensin Converting Enzyme 2, NT (ACE-2, ACEH)

Antibodies / Antibodies-ACE

-20°C

Supplied as a liquid in PBS, 0.02% sodium azide.

Synthetic peptide corresponding to amino acids near the N-terminus of human ACE-2 (Genbank accession NP_068576)

Angiotensin-converting enzyme 2 (ACE2) plays a central role in vascular, renal, and myocardial physiology (1-2). In contrast to its homolog ACE, ACE2 expression is restricted to heart, kidney, and testis. Recently. ACE2 has also been shown to be a functional receptor of the SARS coronavirus (3). The normal function of ACE2 is to convert the inactive vasoconstrictor angiotensin I (AngI) to Ang1-9 and the active form AngII to Ang1-7, unlike ACE, which converts AngI to AngII. While the role of these vasoactive peptides is not well understood, lack of ACE2 expression in ace2-/ace2- mice leads to severely reduced cardiac contractility, indicating its importance in regulating heart function (4).

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml. Human kidney or human testis cell lysate can be used as positive control.
Immunohistochemistry: 2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.