Angiotensin Converting Enzyme 2, Ectodomain (ACE2, ACE-2, ACE-related Carboxypeptidase, Angiotensin Converting Enzyme Homolog, ACEH, DKFZP434A014, EC 3.4.17, OTTHUMP00000022963)

Antibodies / Antibodies-Angiotensin(ogen)

-20°C

Supplied as a lyophilized powder from PBS, 7.4, 5% trehalose. Reconstitute with 500ul sterile PBS.

Recombinant human Angiotensin 1converting enzyme-2 ectodomain aa18-740.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Flow cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml using human, mouse and rat kidney tissue
Flow Cytometry: 0.25ug/10e6 cells using HEK293 human cell line transfected with human ACE2 and eGFP
Immunohistochemistry (Paraffin): 3-15ug/ml using fixed, frozen sectiond of rat lung, immersion fixed paraffin-embedded sections of human kidney, hamster lung, rat lung and rat kidney
Immunoprecipitation: 25ug/ml using conditioned cell culture medium spiked with recombinant human Angiotensin Converting Enzyme 2.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.