Apolipoprotein E (Apolipoprotein-E, Apo E, ApoE, Apo-E, Alzheimer Disease 2, AD2, Apolipoprotein E3, APOE3, Apoprotein, LDLCQ5, LPG, MGC1571)

Antibodies / Antibodies-Lipoproteins

-20°C

Supplied as a lyophilized powder. Reconstitute with 1ml sterile ddH2O. The buffer composition prior to lyophilization is 0.01M sodium phosphate, 0.01M sodium borate, 0.15M sodium chloride, 1% dextran, 1% mannitol.

Human Apolipoprotein E from plasma.

The apolipoproteins are family of amphipathic lipoproteins that plays a crucial role in lipid metabolism. In particular, apolipoprotein E (apo E) is a major component of high density lipoproteins (HDL) chylomicrons and very low-density lipoprotein (VLDL), which functions to remove excess cholesterol. Apo E mediates lipid metabolism and cholesterol homeostasis by binding to several hepatic lipoprotein receptors, such as low density lipoprotein receptor (LDLR) and the LDLR-related protein. Apo E deficiency is linked to hyperlipoproteinemia type III; a genetic disorder characterized by elevated plasma cholesterol and triglyceride levels and accelerated coronary artery disease. Three major isoforms of Apo E (E2, E3, E4) have been identified, with E3 being the most common in human. E4 presence has been associated to increase probability of developing late-onset form of Alzheimer disease.

Applications:
Suitable for use in ELISA, Dot Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:1000 on native and denatured human apoE.
Dot Blot: Native and denatured human apoE.
Immunohistochemistry (Frozen): 1:30
Immunocytochemistry: Cultured human cells
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.