BARD1 (BRCA1 Associated Ring Domain 1)

Antibodies / Antibodies-Cancer Markers

-20°C

Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.05% sodium azide.

Synthetic peptide corresponding to aa50-200 from the internal region of human BARD1.

BARD1 interacts with the N-terminal region of BRCA1. In addition to its ability to bind BRCA1 in vivo and in vitro, BARD1 shares homology with the 2 most conserved regions of BRCA1: the N-terminal ring motif and the C-terminal BRCT domain. The ring motif is a cysteine-rich sequence found in a variety of proteins that regulate cell growth, including the products of tumor suppressor genes and dominant protooncogenes. The BARD1 protein also contains 3 tandem ankyrin repeats. The BARD1/BRCA1 interation is disrupted by tumorigenic amino acid substitutions in BRCA1, implying that the formation of a stable complex between these proteins may be an essential aspect of BRCA1 tumor suppression. BARD1 may be the target of oncogenic mutations in breast or ovarian cancer.

Applications:
Suitable for use in Dot Blot, Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.2-2ug/ml.
Immunoprecipitation: 100-500ug/sample
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.